METHOD FOR RECONSTITUTING A RECOMBINANT PROTEIN TO ITS BIOLOGICALLY ACTIVE FORM
摘要
In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.
申请公布号
WO03031465(A1)
申请公布日期
2003.04.17
申请号
WO2002EP10992
申请日期
2002.10.01
申请人
BOEHRINGER INGELHEIM INTERNATIONAL GMBH;NECINA, ROMAN;SCHLEGL, ROBERT;JUNGBAUER, ALOIS;MACHOLD, CHRISTINE