| 摘要 |
816,372. Chemical testing. WILD, W. Oct. 27, 1955 [Oct. 27, 1954], No. 30821/55. Class 1(1). A chemical pack for examining corpus luteum activity in a woman by determining progesterone or a progesterone metabolite in a body fluid comprises separately packaged reagents of a total volume of not more than 60 ccs., the reagents being provided in such proportions that the determination will be inhibited to the extent of at least 20 per cent of the amount of progesterone or metabolite which corresponds to the normal average during the luteal phase of the menstrual cycle. The reagents specified for determining the sodium salt of pregnanediol glucuronic acid in urine include a mixture of butyl alcohol and amyl alcohol for extracting the metabolite from the urine sample, a mixture of ether and butyl alcohol containing ammonia for adding to water to form a wash liquid for the extract, silica gel impregnated with barium acetate to adsorb and precipitate the barium salt of the metabolite, hydrochloric acid for dissolving the precipitate, a solution of naphthoresorcin in propyl alcohol which reacts on heating to form a coloured complex with the metabolite, and a solution of a green dye in benzene to extract the coloured complex, the green dye serving to mask the colour produced by small amounts of the metabolite. A red to violet colouration indicates a normally active corpus luteum. Apparatus for use with the chemical pack comprises an extraction apparatus in which the urine sample flows from vessel 4 through nozzle piece 3 and extraction tube 2 into lower vessel 1. The extraction tube contains the extracting solvent and is provided with an overflow device 2a to maintain constant the distance between the nozzle and the solvent level. When not in use the parts of the apparatus are stored inside the lower vessel 1 together with two reaction flasks, a flask holder, a shaking cup, a filter, a burner, and a flexible condenser hose. |
