A permanent skin replacement comprising allogenic epithelial cells cultured in vitro and autologous epithelial cells

摘要

Skin replacements to aid wound healing are described. In one embodiment, the living skin replacement is chimeric and is produced by seeding autologous epithelial cells onto a biocompatible substrate containing allogeneic epithelial cells cultured in vitro. The living chimeric skin replacement may be implanted over a permanent dermal replacement previously implanted at the wound site. In another embodiment the skin replacement is a composite comprising an inner, middle and outer component, comprising (a) an inner component comprising a biocompatible dermal construct having a biodegradable or removable scaffold as a base; (b) a middle component comprising epithelial cells; and (c) an outer component comprising epithelial cells cultured in vitro on a dermal construct comprising a dermal portion having a biodegradable or removable scaffold as a base, the dermal portion being combined with a transitional covering and facing inward toward the middle component of epithelial cells. Further described are methods of making the various skin replacements.