| 摘要 |
A method for the in vitro stimulation of immune cells to produce antigen-specific, species-specific monoclonal antibodies comprising: (a) Simulating immune cells from a human or animal in vitro to activate the cells to produce a primary response to an antigen, wherein the antigen is a colloidal metal bound antigen; (b) Immortalising the activated immune cells; and (c) Selecting a monoclonal antibody producing cell. Where the antigen is selected from the group consisting of IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 10, IL-11, IL-12, IL-13 lipid A, phospholipase A2 endotoxins, staphylococcal enterotoxin B and other toxins, Type I interferon, Type II interferon, TNF-, TGF-â, lymphotoxin, migration inhibition factor, CSF monocyte- macrophage CSF, granulocyte CSF, VEGF, angiogenin, heat shock proteins, carbohydrate moieties of blood groups, Rh factors, fibroblast growth factor, inflammatory and immune regulatory proteins, nucleotides, DNA, RNA, mRNA, sense, antisense, polynucleotides, cancer cell specific antigens, mutant p53, tyrosnase, autimmune antigens, immunotherapy drugs, and angiogenic and anti-angiogenic drugs.
|
