发明名称 |
CHARAKTERISIERUNG VON POLYPEPTIDEN DURCH SPALTUNG UND MASSENSPEKTROMETRIE |
摘要 |
Provided is a method for characterizing a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptide fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a difference site from the first cleavage agent; and (e) characterizing the one or more polypeptides in the sample from the fragments identified in steps (c) and (d). |
申请公布号 |
DE69905265(D1) |
申请公布日期 |
2003.03.13 |
申请号 |
DE1999605265 |
申请日期 |
1999.10.01 |
申请人 |
XZILLION GMBH & CO. KG |
发明人 |
SCHMIDT, GUENTER;THOMPSON, HUGIN |
分类号 |
G01N27/62;C07K1/12;C12Q1/02;G01N33/68;(IPC1-7):G01N33/68 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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