摘要 |
<p>The present invention relates to a method of separating a ligand comprised within a mixture comprising the steps of: (a) immobilizing a fusion protein on a matrix, said fusion protein comprising (i) an antibody fragment moiety having a specificity for said ligand and (ii) an affinity domain moiety that attaches to the matrix to form a matrix-fusion protein complex; (b) contacting said mixture with said matrix-fusion protein complex; (c) removing components of said mixture not binding to the antibody fragment moiety of said matrix-fusion protein complex; and (d) eluting said ligand from said matrix-fusion protein complex. The invention furthermore relates to fusion proteins comprising two or more chitin-binding domains, to uses thereof, and to affinity chips and their application.</p> |