| 摘要 |
This invention provides a differential display method for identifying at least one secreted or transmembrane protein comprising the following steps: obtaining mRNA from at least two samples; synthesizing cDNA from said mRNA of each sample; contacting said cDNA from each sample with at least one first primer, said first primer hybridizes to a oligonucleic sequence coding for a leucine-rich motif, and at least one second primer which contains at least ATG sequence, so as to form a cDNA- hybrid molecules; amplifying said cDNA-hybrid molecules, so as to obtain an amplified products; detecting said amplified products; and comparing said amplified products from each sample thereby identifying a distinctive amplified products coding for at least one secreted or transmembrane protein. |
