QUANTIFICATION OF LOW MOLECULAR WEIGHT AND LOW ABUNDANCE PROTEINS USING HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS AND MASS SPECTROMETRY

摘要

Methods are disclosed comprising specific technologies including a system for routinely concentrating proteins from human urine ranging down to approximately 2.5 kDa automated systems for immunosubtraction of major proteins from urine and plasma to reveal minor ones, and systems for routinely fractionating protein mixtures on the basis of native molecular weight, isoelectric point that are applicable to a range of human body fluid proteins, particularly those found in urine.