METHOD OF PREPARING NATIVE SPORE SUSPENSION FOR ANTHRAX VACCINE PREPARING

摘要

FIELD: medicine, microbiology. SUBSTANCE: invention relates to production of anthrax vaccines. Preparing native spore suspension in submerged aerobic culturing vaccine strains of Bacillus anthrax is carried out for two stages: at the first stage culturing is carried out at continuous stirring and aeration with air and maintaining temperature 30-34 C. After termination of spores maturation the second stage is carried out by rise of temperature to 35-38 C and cultural fluid is stirred for short time and periodically. Method allows to increase the content of full-value matured spores in cultural liquid to almost 100%, to determine time of culturing process termination more exactly and to decrease its duration by 2 h. EFFECT: improved method of spore suspension preparing. 3 cl, 2 tbl, 2 ex