METHOD FOR CULTURING ISARIA SINCLAIRII USING DOMESTIC SILKWORM

摘要

PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.