Genome engineering by cell-permeable DNA site-specific recombinases

摘要

The present invention provides polypeptides that contain a site-specific DNA recombinase and a membrane translocation sequence, and nucleic acids that encode such cell-permeable recombinases. The invention also provides methods of stimulating site-specific DNA recombination in cells and in animals using the cell-permeable site-specific DNA recombinases of the invention. Also provided are methods of determining the efficiency of protein transduction into cells; methods of detecting whether site-specific DNA recombination has occurred within a cell; methods of identifying compounds that modulate nuclear metabolism or protein trafficking, uptake, and/or excretion; and methods of identifying peptides that act as membrane translocation signals or that act as nuclear translocation signals or other types of protein targeting signals.