| 摘要 |
<p>A non-human model animal suffering from diseases caused by α1B Ca2+ channel dysfunction which is useful in clarifying various pain transmission mechanisms and methods of inhibiting or treating neurodegenerative diseases, endogenous pain, chronic pain, autonomic diseases, emotional disturbance, etc.; a method of screening a preventive/a remedy for the above diseases with the use of the non-human model animal; and so on. The whole potential-dependent Ca2+ channel α1B gene obtained from a mouse gene library or a fragment of this gene is replaced by a marker gene to thereby construct a targeting vector. After linearizing, the targeting vector is transferred into embryonic stem cells. Then the target embryonic stem cells lacking the potential-dependent Ca2+ channel α1B gene function are microinjected into mouse blastocysts and returned into the womb of a host. Thus the non-human model animal suffering from diseases caused by α1B Ca2+ channel dysfunction is constructed.</p> |
