发明名称 |
Lipidic microparticles linked to multiple proteins |
摘要 |
The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab' fragment attached to a hydrophilic polymer. The present invention further relates to lipidic microparticles with attached proteins which have been first conjugated to linker molecules having a hydrophilic polymer domain and a hydrophobic domain capable of stable association with the microparticle, or proteins which have been engineered to contain a hydrophilic domain and a lipid moiety permitting stable association with the microparticle.
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申请公布号 |
US2003003143(A1) |
申请公布日期 |
2003.01.02 |
申请号 |
US20020177939 |
申请日期 |
2002.06.21 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
PAPAHADJOPOULOS DEMETRIOS;HONG KEELUNG;ZHENG WEIWEN;KIRPOTIN DMITRI B. |
分类号 |
C12N15/09;A61K9/00;A61K9/127;A61K31/7105;A61K31/711;A61K47/10;A61K47/34;A61K47/48;A61K48/00;C07H21/00;C07H21/02;C07H21/04;C12N15/88;(IPC1-7):A61K48/00;C12N15/87 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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