METHOD FOR PRODUCING PTERIDINE COMPOUNDS USING RECOMBINANT ESCHERICHIA COLI

摘要

PURPOSE: A method for producing pteridine compounds using a recombinant Escherichia coli is provided, thereby mass producing the pteridine compounds easily in Escherichia coli. CONSTITUTION: The method for producing pteridine compounds using a recombinant Escherichia coli comprises the steps of: constructing a recombinant vector containing an enzyme gene producing the pteridine compound; transforming Escherichia coli with the recombinant vector to prepare transformed E. coli; and culturing the transformed E. coli, wherein the enzyme gene is selected from GTPCH (GTP cyclohydrolase I) gene, PTPS gene (6-pyruvoyltetrahydropterin synthase), SR gene (sepiapterin reductase), GHluT gene (BH4:UDP-glucose-alpha -glucosyltransferase), Chlorobium tepidum SR gene and pteridine glucotransferase; the pteridine compound is sepiapterin, BH4(6R)-5,6,7,8- tetrahydro-L -biopterin, BH4-alpha -glucoside, L-threo -BH4 and BH4-(alpha or beta)-saccharide; the recombinant vector is pET-GTPCH/PTPS, pET-BH4 or pET-BH4/BGluT; and the transformed E. coli is pET-GTPCH/PTS E. coli(KCTC 1020BP), pET-BH4 E. coli, or pET-BH4/BGluT E. coli.