| 摘要 |
<p>A method of uniformizing DNA fragment contents among DNA fragments contained in a sample which comprises preparing DNA fragments wherein adaptors comprising an oligodeoxyribonucleotide are attached to both ends of DNA fragments in the sample to form restriction enzyme recognition sites not existing in the DNA fragments in the sample and at least the part between the restriction enzyme sites (inclusive) at the both ends is made into double-stranded, denaturing the double-stranded DNA fragments thus prepared, hybridizing the denatured DNA fragments under such conditions that a part of the DNA fragments remain single-stranded, cleaving the hybridized double-stranded DNA fragments with the restriction enzyme having the cleavage site exclusively in the adaptors, and then effecting a PCR using the thus obtained DNA fragments as templates and using primers having base sequences complementary to the base sequences of the adaptors before the cleavage; and a subtraction method involving the step of uniformization by the above-described procedures.</p> |
