Beta -fructofuranosidase and its gene, method of isolating beta -fructofuranosidase gene, system for producing beta -fructofuranosidase, and beta -fructofuranosidase variant

摘要

A novel beta-fructofuranosidase gene and a beta-fructofuranosidase encoded by the gene, a process for isolating a beta-fructofuranosidase gene using the novel beta-fructofuranosidase gene, and a novel beta-fructofuranosidase obtained by this isolation process are disclosed. A novel mold fungus having no beta-fructofuranosidase activity suitable for the production of beta-fructofuranosidase, and a system for producing a recombinant beta-fructofuranosidase using the novel mold fungus as a host is disclosed. Further, a beta-fructofuranosidase variant which selectively and efficiently produces a specific fructooligosaccharide such as 1-kestose from sucrose is disclosed.