发明名称 Detection and quantification of micro-organisms using amplification and restriction enzyme analysis
摘要 In livestock, a number of pathological conditions and/or syndromes exist which may not be attributable to infection by a single organism. In many instances, these pathological conditions and/or syndromes have an effect on the equilibrium that ordinarily exists within the various bacteria comprising the normal bacterial flora. A number of these pathological conditions and/or syndromes have been clinically referred to as dysbacteriosis and bacterial overgrowth. The present invention provides methods for evaluation of the "general health" of livestock by analyzing the composition of microbiological flora. In one aspect, a method of the invention comprises providing a sample of microbiological flora, selectively amplifying nucleic acid in the flora sample, subjecting the amplificate to restriction digestion, and then analyzing the resulting pattern of restriction fragments.
申请公布号 US6495325(B1) 申请公布日期 2002.12.17
申请号 US20000702561 申请日期 2000.10.31
申请人 DR. VAN HAERINGEN LABORATORIUM B.V. 发明人 VAN HAERINGEN HENDRICK;VAN HAERINGEN WILLEM ANNE
分类号 A01K67/00;C12N15/09;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68;C07H21/04 主分类号 A01K67/00
代理机构 代理人
主权项
地址