METHOD FOR DETERMINING THE PEPTIDE HORMONE ACTIVITIES OR THE STEROID HORMONE ACTIVITIES OF A MATERIAL OR SUBSTANCE MIXTURE

摘要

The invention relates to a method for determining the peptide hormone activities or the steroid hormone activities of a material or substance mixture involving the following steps: (a) presenting at least one starting cell with or without endogenous peptide hormone receptors or steroid hormone receptors; (b) transfecting the cell with a specific recombinant reporter gene construct, which has the ability to express a product that is induced and can be measured by the hormone activity of the material; (c) introducing a material to be examined into the transfected cell; (d) producing an easily determinable signal-generating reporter gene translation product through the transfected cell by using the inductive effect of the material on the hormone-reactive promoter situated before the reporter gene; (e) measuring the reporter gene translation product of the transfected cell, and; (f) determining the hormone activity of the material from the measured result.