发明名称 |
SECRETION OF PROTEINS BY ENCAPSULATED CELLS |
摘要 |
The invention provides methods of analyzing a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention furthe r provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.
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申请公布号 |
CA2447691(A1) |
申请公布日期 |
2002.12.05 |
申请号 |
CA20022447691 |
申请日期 |
2002.05.28 |
申请人 |
ONE CELL SYSTEMS, INC. |
发明人 |
MOEN, PHILLIP T., JR.;AKSELBAND, YEVGENYA;MCGRATH, PATRICIA;TRNOVSKY, JAN;JESPERSEN, DIANA |
分类号 |
G01N33/53;C07K16/00;C12P21/08;C12Q1/02;G01N33/543;G01N33/566;G01N33/567;G01N33/569;(IPC1-7):C12Q1/02;C12Q1/24 |
主分类号 |
G01N33/53 |
代理机构 |
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