METHOD FOR ESTIMATING GENOTYPE OF FERTILITY RESTORATIVE GENE LOCUS FOR ORYZA SATIVA BT-TYPE MALE STERILE CYTOPLASM

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting the fertility restorative gene (Rf-1 gene) of Oryza sativa individuals for RT-type male sterile cytoplasm having been utilized for raising hybrid rice. SOLUTION: This method comprises detecting the Rf-1 gene by making use of the fact that a plurality of PCR marker loci present in the proximity of the Rf-1 gene locus link with the Rf-1 gene locus. More specifically, this method comprises easily and accurately investigating the presence/absence of the Rf-1 gene and screening Rf-1 gene homo-type individual(s) by assaying the genotype of the plurality of PCR marker loci present in the proximity of the Rf-1 gene locus by making use of the fact that the Rf-1 gene locus lies between the new PCR marker loci, i.e. S12564 Tsp509I locus and C1361 MwoI locus, present on the Oryza sativa 10th chromosome.