| 摘要 |
PURPOSE: A medium for culturing mammal embryo cells is provided, thereby the mammal embryo cells can be cultured in the medium at a similar growth rate compared to that in vivo, and the medium can be cheaply produced. CONSTITUTION: The medium for culturing mammal embryo cells comprising amino acid, carbohydrate, inorganic salts, trace elements and/or cofactors further comprises synthetic serum, vitamin C, vitamin E, Matrigel as an extract of basal lamina, and cytokine, wherein the amount of the vitamin C is 0.00001 to 0.1 wt.%; the amount of the vitamin E is 0.000001 to 0.01 wt.%; the amount of the Matrigel is 0.01 to 10 wt.%, the amount of the synthetic serum is 1 to 15 % by volume; the amount of the cytokine is 0.01 to 5 wt.%; the cytokine is GM-CSF and/or IL-6; the amount of the GM-CSF (granulocyte-macrophage colony-stimulating factor) is 0.000000002 to 0.00002 wt.%; the amount of the IL-6 (Interleukin-6) is 0.01 to 5 wt.%; the Matrigel is derived from Engelbreth Holm-Swarm mouse sarcoma; and the Matrigel contains laminin comprising collagen IV, heparin sulfate proteoglycans (HSPG), entactin and nidogen, TGF-β (transforming growth factor-beta), fibroblast growth factor, t-PA(tissue plasminogen activator), Engelbreth-Holm-Swarm tumor and other growth factors.
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