METHOD AND REAGENTS FOR INVESTIGATING FUNCTIONAL MOLECULAR INTERACTIONS

摘要

The present invention provides a multiplexed assay for analyzing complex molecular interactions. Thus, the invention relates to a method of identifying, in a single assay, the relative binding of two or more first members of a binding pair to one or more second members of a binding pair. The invention also relates to a method of identifying, in a single assay, the relative modulation, by a modulating agent, of binding between two or more binding pairs or between two or more binding complexes. Further provided are methods of screening, in a single assay, for an agent or agents with a selected binding profile or for an agent that modulates binding between two or more first members of a binding pair and one or more second members of a binding pair or the members of two or more binding complexes. The invention also relates to a set of microspheres coupled with a set of cofactors, wherein the microspheres are labeled with a label specific for each cofactor of the set.