| 摘要 |
<p>A retroviral vector plasmid that contains a retrovirus long terminal repeat, including a U5 region, an R region and a U3 region, which contains a transcriptional regulatory element or a site for inserting such an element; a constitutive transport element, which is heterologous to the U5 region and lacks any translation start codons; and a cloning site, and that substantially lacks any translation start codons between the R region and the cloning site, is provided. In addition, retroviral vector based on the retroviral vector plasmid, and a retroviral vector genome is provided, as is a cell containing the retroviral vector plasmid or retroviral vector. Also provided is a kit containing such a retroviral vector plasmid or retroviral vector. Methods of making and methods of using such a retroviral vector plasmid or retroviral vector also are provided, including, for example, methods of using the vector or vector plasmid to introduce an expressible polynucleotide into a cell such as a neural stem cell.</p> |
