发明名称 |
Highly sensitive gene detection and localization using in situ branched-DNA hybridization |
摘要 |
Methods are provided for highly sensitive and rapid in situ detection of a nucleic acid analyte of a known sequence. The method employs oligonucleotide probes in a series of optimized steps to amplify a signal and decrease background. Sensitivity is enhanced such that the method can detect as few as 1-2 copies of nucleic acid analyte per sample, the sample containing a cell, tissue or similar biological material. Methods of detecting and identifying the position of the nucleic acid analyte in a cell are also provided.
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申请公布号 |
US2002172950(A1) |
申请公布日期 |
2002.11.21 |
申请号 |
US20010872493 |
申请日期 |
2001.06.01 |
申请人 |
KENNY DARYN;SHEN LU PING;ANTAO VINCENT P.;PLAYER AUDREY N.;CAO WEI |
发明人 |
KENNY DARYN;SHEN LU PING;ANTAO VINCENT P.;PLAYER AUDREY N.;CAO WEI |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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