SWAP/COUNTER SELECTION; A RAPID CLONING METHOD

摘要

Disclosed is a method for cloning a sample nucleic acid insert from a donor vector conferring resistance to a first type of antibiotic to an acceptor vector conferring resistance to a second type of antibiotic comprising the method steps of cutting of the vectors with appropriate restriction enzymes yielding ligation capable ends, mixing crude digests without purification, ligating , transforming and selecting transformant clones for the second antibiotic. For excluding false positive resistant clones (from ligation of the two vectors) a chromogenic test for detecting the transformant strains still producing the first antibiotic resistance is performed as a last counter selection step.