| 摘要 |
PURPOSE: A process of preparing glycoprotein by extracting Grifola frondosa belonging to Basidiomycetes. Whereby, the glycoprotein has excellent anticancer activity and can be widely used for the treatment of cancer. CONSTITUTION: Grifola frondosa is soaked in water and extracted at 90 to 100deg.C for 1 to 2hr, and the extract is mixed with alcohol in a ratio of 0.5:1 to 1.5:1(v/v), wherein the alcohol is 90 to 100%(v/v) methanol, ethanol, propanol, butanol or pentanol. For example, 100g dried fruit body of Grifola frondosa is ground with 300ml distilled water, extracted at 95deg.C for 1hr and concentrated under reduced pressure. The extract is added with 95%(v/v) ethanol, left at 4deg.C over night and centrifuged to produce a precipitate, which is dissolved in 20ml distilled water, centrifuged, dialyzed for 3 days and then freeze-dried.
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