发明名称 |
Verfahren zur Herstellung von aktiven Serienproteasen und inaktiven Varianten |
摘要 |
A method for producing biologically active serine proteases, isolated serine protease domains and their amino acid variants in a prokaryotic host is disclosed. The method comprises an N-terminal addition of a helper sequence consisting of a dipeptide which is suitable for degradation by a dipeptidyl amino peptidase, by the expression of the serine protease(s) and/or its (their) fragments containing the N-terminal dipeptide helper sequence, optionally as inclusion bodies, and by the renaturation of the expressed proteins and the activation of the serine protease(s) and/or serine protease domains by splitting off the helper sequence using an exopeptidase. |
申请公布号 |
DE19943177(C2) |
申请公布日期 |
2002.10.24 |
申请号 |
DE1999143177 |
申请日期 |
1999.09.09 |
申请人 |
JENNE, DIETER;MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. |
发明人 |
JENNE, DIETER;WILHARM, ELKE;PARRY, MARINA A.A.;BODE, WOLFRAM;HUBER, ROBERT |
分类号 |
C12N15/09;A61K38/48;C12N1/21;C12N9/48;C12N9/64;C12N15/57;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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