HALOPHILE MICROORGANISM BACILLUS SUBTILIS BS-4 CAPABLE OF PRODUCING HIGH MOLECULAR WEIGHT OF POLY- -GLUTAMIC ACID AND METHOD FOR PRODUCING THE HIGH MOLECULAR WEIGHT OF POLY- -GLUTAMIC ACID USI

摘要

PURPOSE: Provided are halophile microorganism Bacillus subtilis BS-4 capable of producing high molecular weight of poly-γ-glutamic acid and a method for producing the high molecular weight of poly-γ-glutamic acid using the same, thereby producing the high molecular weight poly-γ-glutamic acid in higher yield. CONSTITUTION: The halophile microorganism Bacillus subtilis BS-4 (KCTC 0697BP) capable of producing high molecular weight of poly-γ-glutamic acid is isolated from 'Chug Kook Jang'(Fermented soybean paste) by the steps of: diluting 'Chug Kook Jang' in distilled water; heating the diluted solution at 60 deg. C for 20 minutes; plating the diluted solution on GS agar medium; culturing it at 37 deg. C for 3 days; isolating high viscosity of colonies; culturing the colonies in a broth at 37 deg. C for 20 hours; and selecting a strain showing the best growth activity. The method for producing the high molecular weight of poly-γ-glutamic acid comprises the steps of: (a) culturing the Bacillus subtilis BS-4(KCTC 0697BP) to obtain poly-γ-glutamic acid; and (b) removing polysaccharides from the poly-γ-glutamic acid; dissolving the precipitate of poly-γ -glutamic acid and adding a protease into the solution to decompose an extracellular protein; and dialyzing the solution to remove free glutamic acid and concentrating the solution.