CONCENTRATION AND IDENTIFICATION OF MODERATE-TO-STRONG HITS IN NATURAL PRODUCTS BY CAPILLARY ELECTROPHORESIS

摘要

A method of increasing the concentration of any candidate hit compound (12) in a natural sample that binds to a selected target during capillary electrophoresis, said method comprising the steps of: filling the capillary (10) of a capillary electrophoresis apparatus with a running buffer containing a natural sample; injecting a plug of the selected target (16) into said capillary (10), wherein the concentration of said target (16) is in excess of the concentration of any candidate hit compound (12) in said natural sample; subjecting said target plug to capillary electrophoresis; tracking migration of any said target bound to hit compound (20) at a detection point; collecting said target bound to hit compound detected at said detection point; and isolating said hit compound separate from said target.