| 摘要 |
<p>The invention concerns a method for identifying RNA-binding molecules, comprising the steps of: predicting the structure of an RNA-fragment by an in silico method, choosing a suitable predicted RNA-fragment, synthesizing the cDNA-fragment corresponding to the predicted RNA-fragment, inserting the cDNA-fragment in the upstream proximity of a reporter assay gene, which reporter assay gene produces a signal upon translation, thereby forming a reporter construct, and performing a reporter gene assay, which assay monitors the interaction between a molecule to be tested for RNA-binding and the RNA-fragment of the reporter construct. Furthermore, the invention relates to the use of specific RNA-fragments in the method of the invention.</p> |
