| 摘要 |
<p>The invention relates to biotechnology and can be used for preparation of acrylic and other organic acids. The strain was isolated by the method of step-by-step adaptation from samples of soil taken from chemical plant with the use of acrylonitrile as selection agent. The strain requires neither reach nutrient media nor additional inducers for its cultivation; it can grow on simple hemi-synthetic medium containing acetate as a carbon source and corn extract as a source of additional growth factors. Strain C-32 was relates to species denitrificans of genera Alcaligenes on the base of its morphological, culture, and biochemical properties. Strain Alcaligenes denitrificans C-32 VKM B-2243 D possesses high nitrilase activity on acrylonitrile [555.6 mmole/(h*g) at 30 °C]. Maximum nitrilase activity of cells of the strain [1606.0 mmole/h*g)] occurs at 65 °C. Enzymatic activity of the strain appears in a wide pH range (4 to 12). 3 Tables are enclosed.</p> |
