发明名称 |
RECOVERY OF ENZYME ACTIVITY FROM ENVELOPED VIRUSES |
摘要 |
A method of recovering an enzyme activity, such as reverse transcriptase (RT) activity, from enveloped viruses, such as HIV, in a biological sample containing a non-protected enzymes, is described. A cysteine-modifying substance is used to destroy the activity of the non-protected enzymes, followed by removal of the enveloped virus particles or inactivation of the cysteine-modifying substance with a chemical. Then the virus envelope is lysed and the released enzymes are recovered. Commercial packages containing written and/or data carrier instructions and some chemicals for performing laboratory steps for recovery of an enzyme activity from enveloped viruses, are also disclosed.
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申请公布号 |
WO02082088(A1) |
申请公布日期 |
2002.10.17 |
申请号 |
WO2002SE00612 |
申请日期 |
2002.03.27 |
申请人 |
CAVIDI TECH AB;KAELLANDER, CLAS;GRONOWITZ, SIMON;GATU, TOMMY |
发明人 |
KAELLANDER, CLAS;GRONOWITZ, SIMON;GATU, TOMMY |
分类号 |
C12N9/00;C12N9/12;(IPC1-7):G01N33/573;C12Q1/70;C12Q1/48 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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