GANGLIOSIDE-ASSOCIATED RECOMBINANT ANTIBODIES AND THE USE THEREOF IN THE DIAGNOSIS AND TREATMENT OF TUMOURS

摘要

The invention relates to the obtention of genetically-engineered antibodies from the P3 (AcM P3) murine monoclonal antibody, which is produced by the hybridoma deposited under number ECACC 94113026 in accordance with the Budapest treaty, and from the 1E10 (AcMai 1E10) anti-idiotype thereof, which is produced by hybridoma ECACC 97112901, with the purpose of obtaining antibodies which have the same recognition properties as the originals but which are less immunogenic. The chimaeric antibodies contain the variable domains of murine immunoglobulin and the constant regions of human immunoglobulin. The humanised antibodies, in addition to containing the constant regions of human immunoglobulin, are modified in the murine frameworks (FRs) region and, in particular, in those areas that can be converted into a T-cell antigenic site, as a result of which some FRS positions are human. Said antibodies can be used for the diagnosis and thera py of different types of tumours.