产生CR1多肽的方法

摘要

本发明涉及C3b/C4b受体(CR1)基因及其编码蛋白质。还涉及含70核苷酸的CR1核酸顺序及其片段和其含24氨基酸的编码肽或蛋白质。本发明还提供了CR1蛋白质及其片段的表达。本发明的基因和蛋白质用于包括补体活性、各种免疫系统或炎性疾病的疾病诊断和治疗。本发明实施例叙述全长CR1 cDNA及其片段的克隆、核苷酸顺序及推断的氨基酸顺序以及CR1蛋白质及其片段的表达。获得含C3b和(或)C4b结合位点和呈因子1-辅因子活性的CR1蛋白质及其片段的表达。

主权项

1.一种产生全长CR1蛋白的方法，其特征在于，它包括：培养用表达载体转化的细菌宿主细胞，该表达载体表达氨基酸序列如下的蛋白： QCNAPEWLP FARPTNLTDE FEFPIGTYLN YECRPGYSGR PFSIICLKNS VWTGAKDRCRRKSCRNPPDP VNGMVHVIKG IQFGSQIKYS CTKGYRLIGS SSATCIISGD TVIWDNETPICDRIPCGLPP TITNGDFIST NRENFHYGSV VTYRCNPGSG GRKVFELVGE PSIYCTSNDDQVGIWSGPAP QCIIPNKCTP PNVENGILVS DNRSLFSLNE VVEFRCQPGF VMKGPRRVKCQALNKWEPEL PSCSRVCQPP PDVLHAERTQ RDKDNFSPGQ EVFYSCEPGY DLRGAASMRCTPQGDWSPAA PTCEVKSCDD FMGQLLNGRV LFPVNLQLGA KVDFVCDEGF QLKGSSASYCVLAGMESLWN SSVPVCEQIF CPSPPVIPNG RHTGKPLEVF PFGKAVNYTC DPHPDRGTSFDLIGESTIRC TSDPQGNGVW SSPAPRCGIL GHCQAPDHFL FAKLKTQTNA SDFPIGTSLKYECRPEYYGR PFSITCLDNL VWSSPKDVCK RKSCKTPPDP VNGMVHVITD IQVGSRINYSCTTGHRLIGH SSAECILSGN AAHWSTKPPI CQRIPCGLPP TIANGDFIST NRENFHYGSVVTYRCNPGSG GRKVFELVGE PSIYCTSNDD QVGIWSGPAP QCIIPNKCTP PNVENGILVSDNRSLFSLNE VVEFRCQPGF VMKGPRRVKC QALNKWEPEL PSCSRVCQPP PDVLHAERTQRDKDNFSPGQ EVFYSCEPGY DLRGAASMRC TPQGDWSPAA PTCEVKSCDD FMGQLLNGRVLFPVNLQLGA KVDFVCDEGF QLKGSSASYC VLAGMESLWN SSVPVCEQIF CPSPPVIPNGRHTGKPLEVF PFGKAVNYTC DPHPDRGTSF DLIGESTIRC TSDPQGNGVW SSPAPRCGILGHCQAPDHFL FAKLKTQTNA SDFPIGTSLK YECRPEYYGR PFSITCLDNL VWSSPKDVCKRKSCKTPPDP VNGMVHVITD IQVGSRINYS CTTGHRLIGH SSAECILSGN TAHWSTKPPICQRIPCGLPP TIANGDFIST NRENFHYGSV VTYRCNLGSR GRKVFELVGE PSIYCTSNDDQVGIWSGPAP QCIIPNKCTP PNVENGILVS DNRSLFSLNE VVEFRCQPGF VMKGPRRVKCQALNKWEPEL PSCSRVCQPP PEILHGEHTP SHQDNFSPGQ EVFYSCEPGY DLRGAASLHCTPQGDWSPEA PRCAVKSCDD FLGQLPHGRV LFPLNLQLGA KVSFVCDEGF RLKGSSVSHCVLVGMRSLWN NSVPVCEHIF CPNPPAILNG RHTGTPSGDI PYGKEISYTC DPHPDRGMTFNLIGESTIRC TSDPHGNGVW SSPAPRCELS VRAGHCKTPE QFPFASPTIP INDFEFPVGTSLNYECRPGY FGKMFSISCL ENLVWSSVED NCRRKSCGPP PEPFNGMVHI NTDTQFGSTVNYSCNEGFRL IGSPSTTCLV SGNNVTWDKK APICEIISCE PPPTISNGDF YSNNRTSFHNGTVVTYQCHT GPDGEQLFEL VGERSIYCTS KDDQVGVWSS PPPRCISTNK CTAPEVENAIRVPGNRSFFS LTEIIRFRCQ PGFVMVGSHT VQCQTNGRWG PKLPHCSRVC QPPPEILHGEHTLSHQDNFS PGQEVFYSCE PSYDLRGAAS LHCTPQGDWS PEAPRCTVKS CDDFLGQLPHGRVLLPLNLQ LGAKVSFVCD EGFRLKGRSA SHCVLAGMKA LWNSSVPVCE QIFCPNPPAILNGRHTGTPF GDIPYGKEIS YACDTHPDRG MTFNLIGESS IRCTSDPQGN GVWSSPAPRCELSVPAACPH PPKIQNGHYI GGHVSLYLPG MTISYTCDPG YLLVGKGFIF CTDQGIWSQLDHYCKEVNCS FPLFMNGISK ELEMKKVYHY GDYVTLKCED GYTLEGSPWS QCQADDRWDPPLAKCTSRAH DALIVGTLSG TIFFILLIIF LSWIILKHRK GNNAHENPKE VAIHLHSQGGSSVHPRTLQT NEENSRVLP。