Use of an histone deacetylase inhibitor for the treatment of diseases associated with an hpv infection

摘要

In the preparation of a medicament for the treatment of diseases associated with an human papillomavirus (HPV) infection histone deacetylase inhibitor is used. ACTIVITY : Cytostatic; Dermatological; Virucide. MECHANISM OF ACTION : Cell growth inhibitor; Cell proliferation inhibitor. To evaluate the effect of sodium butyrate (histone deacetylase) (HDAC) inhibitor on HPV16/18-induced carcinogenesis, the HPV18-positive cervical carcinoma cell line HeLa as well as primary human foreskin keratinocytes, which were separately immortalized with amphotropic retroviruses carrying the open reading frames of HPV16 E6, E7 or E6/E7 were used. To detect the effect of HDAC on cell proliferation, flow cytometric analysis on cellular DNA was carried out. It was observed that the treatment with sodium butyrate (6 mM) for 6 hours resulted in a significant increase of G1 fraction (from 56.8% to 75.7%) whereas the number of cells in S-phase was diminished from 24.9% to 8.5%.