发明名称 |
SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS |
摘要 |
A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA. |
申请公布号 |
WO02077246(A2) |
申请公布日期 |
2002.10.03 |
申请号 |
WO2002EP03266 |
申请日期 |
2002.03.22 |
申请人 |
ICON GENETICS AG;ICON GENETICS, INC.;KLIMYUK, VICTOR;GLEBA, YURI;MARILLONNET, SYLVESTRE |
发明人 |
KLIMYUK, VICTOR;GLEBA, YURI;MARILLONNET, SYLVESTRE |
分类号 |
A01H5/00;C12N1/21;C12N5/10;C12N7/00;C12N15/09;C12N15/82 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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