SCREENING METHOD COMPRISING COMBINATION OF DISCRETE DIMENSION ASSAY TESTING OF ACTIVES, GENE MUTATION AND APPLICATION ASSAY TESTING OF SELECTED ACTIVES

摘要

The present invention relates to a screening method for identifying actives (peptides or proteins) with improved performance comprising: (1) screening a library of actives encoded by nucleotide sequences in at least two different discrete dimension assays employing selected parameters and selecting the actives exhibiting improved properties in the discrete dimension assays, (2) collecting nucleotide sequences encoding the selected actives, (3) mutating collected nucleotide sequences, (4) screening actives encoded by the mutated nucleotide sequences in an application assay and selecting actives, which exhibit improved performance.