| 摘要 |
<p>A novel gene defining a novel human UDP GlcNAc: Galbgr;1 3GAlNAcagr; bgr;1, 6GlcNAc transferase, termed C2GnT3, with unique enzymatic properties is disclosed. The invention discloses isolated DNA molecules and DNA constructs encoding C2GnT3, as well as cloning and expression vectors including such DNA. The enzyme C2GnT3 active derivatives thereof are disclosed, in particular soluble derivatives comprising the catalytically active domain of C2GnT3. Further, the invention discloses methods of obtaining 1,6 N acetylglucosaminyl glycosylated saccharides, glycopeptides or glycoproteins by use of an enzymatically active C2GnT3 protein or fusion protein thereof or by using cells stably transfected with a vector including DNA encoding an enzymatically active C2GnT3 protein. Methods are disclosed for the identification of agents with the ability to inhibit or stimulate the biological activity of C2GnT3.</p> |
