发明名称 |
NON-TOXIC MUTANTS OF PATHOGENIC GRAM-NEGATIVE BACTERIA |
摘要 |
<p>A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB ge ne of a gram-negative bacterial pathogen so that there is a lack of a functiona l htrB protein, resulting in a mutant that lacks one or more secondary acyl chains contained in the wild type gram-negative bacterial pathogen, and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, the endotoxin isolated therefrom, or the endotox in isolated therefrom which is then conjugated to a carrier protein, to immuniz e an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.</p> |
申请公布号 |
CA2238640(C) |
申请公布日期 |
2002.09.17 |
申请号 |
CA19962238640 |
申请日期 |
1996.11.27 |
申请人 |
UNIVERSITY OF IOWA RESEARCH FOUNDATION;THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;AMERICAN CYANAMID COMPANY |
发明人 |
APICELLA, MICHAEL A.;SUNSHINE, MELVIN G.;LEE, NA-GYONG;ARUMUGHAM, RASAPPA;GIBSON, BRADFORD W. |
分类号 |
C12N15/09;A61K39/00;A61K39/02;A61K39/09;A61K39/095;A61K39/102;A61K39/104;A61K39/106;A61K39/112;A61K39/116;A61P31/04;C12N1/21;C12N9/10;C12N15/01;C12R1/01;(IPC1-7):C12N1/20;C07K16/12;A23K1/16;C12N1/36;A61K39/385;A61K39/40;G01N33/569 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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