| 摘要 |
PROBLEM TO BE SOLVED: To reduce bulk effects generated by refractive index change of a solvent for enhance measuring precision of a coupling condition between a sensing substance and a specimen, when a sample solution being supplied to a measuring unit, in a measuring instrument using attenuation of total reflection by surface plasmon resonance or the like. SOLUTION: The sample solution 15, comprising the specimen and the solvent is supplied dropingwisely to the measuring unit 10 with the sensing substance 14 fixed thereon by a sample supply mechanism 70, a light beam 30 is made incident at various angles to provide a total reflection condition in an interface between a metal film formed in the inner bottom face of the measuring unit 10 and a dielectric block thereunder, the light beam 30 total-reflected in the interface is detected by a photodetector 40, and change with the lapse of time in the attenuation angle of the total reflection is found, based on the photodetection value to measure the coupling condition between the sensing substance 14 and the specimen. Since the solvent same to the solvent contained in the sample solution 15 is supplied preliminarily to an inside of the measuring unit 10 by a solvent supply mechanism 80, the refractive index of the solvent will not vary, when the sample solution 15 is supplied to the unit 10.
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