发明名称 BIOSCOPY METHOD AND METHOD OF NUCLEIC ACID AMPLIFICATION
摘要 A bioscopy method whereby life or death of cells and/or bacteria is judged by applying nucleic acid quantification; and a method of nucleic acid amplification whereby cells and/or nucleic acid genes are efficiently and directly amplified. For example, DNA stable and unstable parts in samples containing viable cells and dead cells at different mixing ratios are amplified by PCR. Then PCR products are observed in a sample A wherein the DNA unstable part comprises viable cells exclusively while no PCR product is observed in a sample H wherein the DNA unstable part comprises dead cells exclusively. Thus, the life/death and mixing ratios of the samples can be understood.
申请公布号 WO02052034(A8) 申请公布日期 2002.08.29
申请号 WO2001JP11489 申请日期 2001.12.26
申请人 OSHIMA, JOJI 发明人 OSHIMA, JOJI
分类号 C12Q1/68;(IPC1-7):C12Q1/04;C12N15/10 主分类号 C12Q1/68
代理机构 代理人
主权项
地址