A method for purifying an RNA virus comprising generating a clonal virus and purifying the clonal virus by ultracentrifugation without pelleting

摘要

Method of purifying and using such viruses that are able to replicate and thereby kill neoplastic cells with a deficiency in the IFN-mediated antiviral response. The use in treating neoplastic disease including cancer and large tumours are also described. RNA and DNA viruses are useful in this regard. The method describes a method of purifying an RNA by initially plaque purifying to generate a clonal virus, inoculating eggs with the clonal virus, incubating the eggs, chilling the eggs, harvesting allantoic fluid from the eggs, removing cell debris from the allantoic fluid prior to the ultracentrafugation step.