| 摘要 |
The present invention relates in general to the pseudotyping of retroviruses with lymphocytic choriomeningitis virus. In particular, the invention relates to pseudotyping in MLV packaging cells which are optionally env-deleted, or in packaging cells derived from lentiviruses. Preferably, pseudotyping takes place by infection with LCMV or a preferably env-deleted mutant, or by transfection with an expression plasmid containing the gp gene of LCMV or a part thereof and optionally, in addition, the np, 1 and/or the z gene of LCMV. The invention also relates to the use of such pseudotypes for the infection of cells, particularly the use in gene therapy.
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