发明名称 |
DNA POLYMERASE HAVING ABILITY TO REDUCE INNATE SELECTIVE DISCRIMINATION AGAINST FLUORESCENT DYE-LABELED DIDEOXYNUCLEOTIDES |
摘要 |
The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases. |
申请公布号 |
EP1115848(A4) |
申请公布日期 |
2002.08.21 |
申请号 |
EP19990900592 |
申请日期 |
1999.01.14 |
申请人 |
SHANGHAI INSTITUTE OF BIOCHEMISTRY CHINESE ACADEMY OF SCIENCES |
发明人 |
HONG, GUOFAN;HUANG, WEI-HUA |
分类号 |
C12N15/09;C07H21/04;C12N1/21;C12N9/12;C12N15/00;C12N15/55;C12Q1/68;C12R1/07;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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