发明名称 |
Method of detecting a pathogen using a virus |
摘要 |
A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).
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申请公布号 |
US6436652(B1) |
申请公布日期 |
2002.08.20 |
申请号 |
US19990376288 |
申请日期 |
1999.08.18 |
申请人 |
HER MAJESTY THE QUEEN IN RIGHT OF CANADA, AS REPRESENTED BY THE MINISTER OF NATIONAL DEFENCE OF HER MAJESTY'S CANADIAN GOVERNMENT |
发明人 |
CHERWONOGRODZKY JOHN W.;LOTFALI KAMIL |
分类号 |
A61K38/54;A61K39/12;C12Q1/70;G01N33/554;G01N33/563;G01N33/569;(IPC1-7):G01N33/554 |
主分类号 |
A61K38/54 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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