发明名称 |
Method of differentiating beer yeast |
摘要 |
<p>The method of differentiating beer yeast of the invention is a method which comprises a first step of synthesizing a primer capable of amplifying the linker portion between a base sequence (A) and a base sequence (B) in a novel gene (C) which has the base sequence (B) comprising a portion of yeast chromosome IX linked downstream from the base sequence (A) comprising a portion of the N-terminal end of yeast gene Lg-FLO1, and which includes the base sequences listed as SEQ. ID. Nos. 1-6 of the Sequence Listing; a second step of carrying out a PCR (Polymerase Chain Reaction) using the primer synthesized in the first step and DNA separated from a yeast specimen; and a third step of differentiating whether the yeast is bottom-fermenting yeast or wild yeast, based on the PCR amplification product obtained from the second step.</p> |
申请公布号 |
EP1231283(A2) |
申请公布日期 |
2002.08.14 |
申请号 |
EP20020002058 |
申请日期 |
2002.02.08 |
申请人 |
SAPPORO BREWERIES LTD. |
发明人 |
SATO, MASAHIDE;TSUCHIYA, YOUICHI |
分类号 |
C12N15/09;C12Q1/68;C12Q1/04;C12R1/85;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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