| 摘要 |
<p>A method of determining the antigens encoded by a genomic or cDNA library is disclosed. Dendritic or other antigen presenting cells are transfected with DNA fragments in a vector which includes a signal peptide coding sequence and an sequence which encodes a peptide binding to a receptor on the antigen presenting cell. The expressed DNA fragments are secreted under control of the signal peptide, and bind to a cell surface receptor. The antigen presenting cells are used to generate monoclonal antibodies. The monoclonal antibodies may be screened by cloning the same fragments into a display vector containing a transmembrane domain thereby displaying the expressed proteins on the surface of a host cell. The monoclonal are screened against these displayed proteins for a positive match.</p> |
