Methods for tissue culturing and transforming elite inbreds of Zea mays L.

摘要

The present invention is directed to methods for the tissue culture and transformation of elite inbreds of corn (Zea mays L.). More specifically, the present invention is directed to a method for initiating Type II callus from corn tissue. The present invention is also directed to a method for enhancing the intergration of foreign DNA in the transformation of corn using a heat shock treatment. The present invention is further directed to a method of transforming elite inbreds of corn using Agrobacterium.