| 摘要 |
PURPOSE: To measure enzyme activity via the infrared spectroscopic method by immobilizing a substrate to the surface of an attenuated total reflection prism directly or indirectly. CONSTITUTION: A substrate is immobilized directly or indirectly to the surface of an attenuated total reflection prism 1, and a sample 4 is introduced to start reaction between the substrate and enzyme contained in the sample 4, thereby obtaining two infrared absorption spectra in different reaction time per sample. Then, the concentration change of the substrate or an enzyme reaction product in the sample 4 per unit time is calculated from the spectra, thereby measuring enzyme activity in the sample 4. |
