New DNA-methyltransferase M.EacI, useful for protecting double stranded DNA against cleavage by restriction enzymes, derived from Eubacterium acidaminophilum

摘要

DNA-methyltransferase M.EacI from Eubacterium acidaminophilum. The sequence encoding M.EacI of 1929 base pairs (bp), and the derived protein sequence, 642 amino acids, are fully defined in the specification. An Independent claim is also included for preparation of M.EacI by biochemical isolation from E. acidaminophilum or by overexpressing the coding sequence in Escherichia coli.